Genomic and biological characterization of Pigeon Paramyxovirus Type-1 isolated from Indian pigeons: First report on the six nucleotide insertion in the non-coding region of nucleoprotein gene

Genotypic analysis of Pigeon Paramyxovirus Type-1 (PPMV-1) virus, in corroboration with pathotyping, underpins the knowledge on occurrence PPMV-1 mediated Newcastle disease poultry. In present study, two isolates, namely D167 and D168, obtained from feral pigeons India year 2014 were analysed for their genotypic pathotypic characteristics. Phylogenetic Fusion (F) gene grouped D168 under sub-genotypes VI.2.2.2. VI.2.1.1.2.2. respectively. The separate positioning these isolates within genotype VI is corroborated variation restriction enzyme sites through virtual mapping. Analysis FPCS region showed that both possessed 112RRQKRF117 amino-acid pattern. Intra-cerebral pathogenicity index (ICPI) was 1.57 1.3, mean death time (MDT) 48 70 hrs respectively, designating as a mesogenic pathotype, velogenic pathotype. Histopathological proventriculus, intestine, lungs spleen chickens infected intramuscular (I/M), subcutaneous (S/C) oculo-nasal/oral (O/N) routes inoculation similar severity tissue damage. This study shows belonging to different without common ancestor, can occur together particular geographical region. It also gains virulence chickens, which needs be addressed order avoid emergence new variant causing chickens.